Research & Development

/Research & Development

Oocyte-free reproduction by embryo bioprinting: Current advanced reproductive techniques, such as in-vitro or in-vivo fertilization and somatic cell nuclear transfer require a large number of high-quality, matured oocytes to produce a few healthy offsprings. However, obtaining such a large number of good quality oocytes is a challenging task in most of the mammalian species. Occasionally, the oocyte collection procedure inflict serious, irreversible injuries to the donor animals.

As a solution to this problem, our novel approach, oocyte-free preimplantation embryo production by bioprinting, does not require eggs from female animals. In this approach, Embryonic Stem Cells (ESC) and/or induced Pluripotent Stem Cells (iPSC) generated from the animal of interest are the major biomaterial required to de-novo bioprint embryos. This method helps in production of true clones of a donor animal with 100% genomic identity including mitochondrial genome, and production of a large number of identical offsprings from a single embryo (monozygotic multiples).

Extraembryonic shell of a preimplantation embryo is bioprinted using Trophoblast Stem Cells, and Extraembryonic and Visceral Endoderm Cells. These shells can be used as the general structure across different breeds and strains within a species. By depositing pluripotent stem cells derived from the animal of interest inside these shells, cloned offsprings can be produced.

Bird cloning: Hatching out sex selected baby chicks are the pressing need for todays poultry industry to avoid maceration or gassing of billions of male layer and female broiler chicks each year. Taken this necessity as an opportunity, GametoGeneTech actively works towards perfecting a NOVEL METHODOLOGY TO CLONE AVIAN SPECIES. Our comprehensive understanding on the eukaryotic cell cycle mechanisms and capability to generate chicken stem cells have given the possibility to modify day-old chicken embryos (freshly laid fertilized eggs) to our favour. The new method is designed to be more cost-effective and produce a large number of true clones of a donor bird at a rapid phase.